Streptavidin-HRP from Cell Signaling Technology

Product Specs
ItemStreptavidin-HRP
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number3999S
Quantity1 ml
ApplicationsW
Conjugate/Tag/LabelHRP
Research AreaRelated Products
Molecule NameStreptavidin
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Unveiling the role of PUS7-mediated pseudouridylation in host protein interactions specific for the SARS-CoV-2 RNA genomeMolecular Therapy - Nucleic AcidsDecember 12, 2023Roberto Giambruno, Elsa Zacco, Camilla Ugolini, Andrea Vandelli, Logan Mulroney, Manfredi D’Onghia, Bianca Giuliani, Elena Criscuolo, Matteo Castelli, Nicola Clementi, et al.Addgene_182925). The following antibodies were used in this study: streptavidin-horseradish peroxidase (Cat. #3999) from Cell Signaling Technology. Anti-vinculin (Clone H Vin 1 0.2 ML; Cat. #V9131) from Merck. Anti-HA-11 epitope tag

(2) Cancer-specific glycosylation of CD13 impacts its detection and activity in preclinical cancer tissuesiScienceNovember 17, 2023Francis M. Barnieh, Sebastian P. Galuska, Paul M. Loadman, Simon Ward, Robert A. Falconer, Sherif F. El-Khamisy[EPR4059],Abcam,Cat# ab108382.RRID: AB_10890797 CD13 antibody [ab236283],Abcam,Cat# ab236283 Streptavidin-HRP [3999],Cell Signaling Technology,Cat# 3999.RRID: AB_10830897 "Anti-rabbit IgG, HRP-linked Antibody [7074]",Cell Signaling Technology

(3) Chemical Acetylation of Ligands and Two-Step Digestion Protocol for Reducing Codigestion in Affinity Purification–Mass SpectrometryJournal of Proteome ResearchOctober 6, 2023David M. Hollenstein, Margarita Maurer-Granofszky, Wolfgang Reiter, Dorothea Anrather, Thomas Gossenreiter, Riccardo Babic, Natascha Hartl, Claudine Kraft, Markus Hartlmouse monoclonal anti-Pgk1 antibody (1:10 000; 22C5D8, Invitrogen), and Streptavidin-HRP (1:3000; #3999, Cell Signaling Technology). Standard Biochemical Assays Yeast cell extracts were incubated with urea

(4) NADPH oxidase 1: A target in the capacity of dimeric ECG and EGCG procyanidins to inhibit colorectal cancer cell invasionRedox BiologySeptember 1, 2023Wei Zhu, Patricia I. Oteizasecondary antibodies anti-rabbit HRP-conjugated (#7074), anti-rabbit biotinylated (#14708), streptavidin (#3999) and the biotinylated ladder (#7727) were from Cell Signaling Technology, Inc. (Danvers, MA). The primary

(5) FOXA1 O-GlcNAcylation–mediated transcriptional switch governs metastasis capacity in breast cancerScience AdvancesAugust 18, 2023Yajie Liu, Kairan Yu, Xiaotian Kong, Keren Zhang, Lingyan Wang, Nana Zhang, Qiushi Chen, Mingshan Niu, Wenli Li, Xiaomin Zhong, et al.(CST, #7074, 1:20,000), anti-mouse IgM-HRP (Abcam, #ab97230, 1:20,000), and Streptavidin-HRP (CST, #3999, 1:50,000). Because of the high expression levels of HA-FOXA1 variants in stably transfected breast cancer

(6) A Turn-On Fluorescent Amino Acid Sensor Reveals Chloroquine’s Effect on Cellular Amino Acids via Inhibiting Cathepsin LACS Central ScienceMay 24, 2023Michael R. Smith, Le Zhang, Yizhen Jin, Min Yang, Anusha Bade, Kevin D. Gillis, Sadhan Jana, Ramesh Naidu Bypaneni, Timothy E. Glass, Hening Lin(sc-2003) were purchased from Santa Cruz. Antibodies against LC3 (Cat. #12741) and Streptavidin-HRP (Cat. #3999) were purchased from Cell Signaling Technology. Antibodies against PSAP (A1819) and NPC2 (A5413) were

(7) Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signalingMolecular CellMay 18, 2023Edoardo José Longarini, Helen Dauben, Carolina Locatelli, Anne R. Wondisford, Rebecca Smith, Charlotte Muench, Andreas Kolvenbach, Michelle Lee Lynskey, Alexis Pope, Juan José Bonfiglio, et al.(HRP),Sigma-Aldrich,Cat# A8592; RRID:AB_439702 Anti-Streptavidin antibody (HRP),Cell Signaling Technology,Cat# 3999; RRID:AB_10830897 Anti-GST antibody (HRP),Abcam,Cat# ab3416; RRID:AB_303783 Anti-mono/poly-ADP0-ribose

(8) African swine fever virus QP383R dampens type I interferon production by promoting cGAS palmitoylationFrontiers in ImmunologyMay 9, 2023Siyuan Hao, Xiaojie Zheng, Yingqi Zhu, Yao Yao, Sihan Li, Yangyang Xu, Wen-hai Feng(#4947), mouse IκBα mAb (#4814), and rabbit p-IκBα (Ser 32) mAb (#2859). Streptavidin-HRP antibody (#3999) were acquired from Cell Signaling Technology (Boston, MA, USA). The rabbit cGAS pAb (HA500023) was

(9) Integrative multi-omics and drug-response characterization of patient-derived prostate cancer primary cellsSignal transduction and targeted therapyMay 1, 2023Wang Z, Li Y, Zhao W, Jiang S, Huang Y, Hou J, Zhang X, Zhai Z, Yang C, Wang J, et al.Millipore). Subsequently, the membrane was treated overnight at 4 °C with HRP-labeled streptavidin (CST, 3999) diluted 1:4000 in blocking buffer. ChemiScope S6 system was used to visualize the target proteins. To

(10) Structural basis for botulinum neurotoxin E recognition of synaptic vesicle protein 2Nature CommunicationsApril 24, 2023Zheng Liu, Pyung-Gang Lee, Nadja Krez, Kwok-ho Lam, Hao Liu, Adina Przykopanski, Peng Chen, Guorui Yao, Sicai Zhang, Jacqueline M. Tremblay, et al.HCE was detected with Streptavidin-tagged horseradish peroxidase (HRP) (Cell signaling technology, 3999s), which recognizes biotinylated HCE. All experiments were repeated three times independently. Entry

(11) The association of UBAP2L and G3BP1 mediated by small nucleolar RNA is essential for stress granule formationCommunications BiologyApril 14, 2023Eri Asano-Inami, Akira Yokoi, Mai Sugiyama, Toshinori Hyodo, Tomonari Hamaguchi, Hiroaki Kajiyamaanti-eIF2α (5324), anti-phospho-eIF2α(3398), anti-FXR1 (12295),anti-FMRP (4317), and anti-streptavidin-HRP (3999) antibodies were obtained from Cell Signaling (Danvers, CA, USA); anti-eIF4E (sc-9976) and anti-PABPC1

(12) Selenoprotein K enhances STING oligomerization to facilitate antiviral responsePLoS pathogensApril 1, 2023Lv L, Chai L, Wang J, Wang M, Qin D, Song H, Fu Y, Zhao C, Jia J, Zhao W, et al.#4947), anti-p-STAT1 (Tyr701; #9167), anti-cGAS (#31659), anti-HA (#3724), anti-streptavidin-HRP (#3999), and anti-rabbit IgG (#2729) antibodies were purchased from Cell Signaling Technology. Anti-SELENOK

(13) An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin loopingNature communicationsMarch 3, 2023Wang Z, Liang Q, Qian X, Hu B, Zheng Z, Wang J, Hu Y, Bao Z, Zhao K, Zhou Y, et al.milk, incubated with streptavidin labeled with horseradish peroxidase (HRP; Cell Signaling Technology, 3999), and then visualized by a Protein Imaging System (BIO-RAD, ChemiDoc XRS+). The oligonucleotide sequences

(14) A TurboID-based proximity labelling approach for identifying the DNA-binding proteinsSTAR ProtocolsMarch 1, 2023Xia-fei Wei, Shan Li, Jie-li HuAntibody,Beyotime,Cat#AG019;RRID:AB_2861160 Streptavidin-HRP antibody,Cell Signaling Technology,Cat#3999;RRID:AB_10830897 ,, Bacterial and virus strains,Bacterial and virus strains,Bacterial and virus strains ,, DH5α

(15) Myristic acid as a checkpoint to regulate STING-dependent autophagy and interferon responses by promoting N-myristoylationNature communicationsFebruary 7, 2023Jia M, Wang Y, Wang J, Qin D, Wang M, Chai L, Fu Y, Zhao C, Gao C, Jia J, et al.#4302), anti-p-STAT1 (1:1000, #9167), anti-cGAS (1:1000, #31659), and anti-streptavidin-HRP (1:1000, #3999) were purchased from Cell Signaling Technology. Anti-NMT1 (1:2000, ab186123), anti-ICP5 (1:3000, ab6508),

(16) Periostin Protects Against Alcohol-related Liver Disease by Activating Autophagy by Interacting With Protein Disulfide IsomeraseCellular and molecular gastroenterology and hepatologyJanuary 1, 2023Zhang Y, Jin J, Wu H, Huang J, Ye S, Qiu J, Ouyang G, Wu T, Liu F, Liu Y.by Western blot. The biotinylated proteins were detected by anti-HA (3724S; CST) and HRP-streptavidin (3999S; CST) antibodies. Meanwhile, the remaining supernatant was further incubated with 80 μl NeutrAvidin

(17) Mapping of the podocin proximity-dependent proteome reveals novel components of the kidney podocyte foot processFrontiers in cell and developmental biologyJanuary 1, 2023Gerlach GF, Imseis ZH, Cooper SL, Santos AN, O'Brien LL.[1:500], rabbit anti-podocin (Invitrogen, PA5-79757) [1:500], and streptavidin-HRP (Cell Signaling, 3999S) [1:1000] were applied in 3% BSA + TBST. The membrane and primary antibodies were allowed to incubate

(18) A versatile design platform for glycoengineering therapeutic antibodiesmAbsDecember 31, 2022Seth D. Ludwig, Zachary J. Bernstein, Christian Agatemor, Kris Dammen-Brower, Jeffrey Ruffolo, Jonah M. Rosas, Jeremy D. Post, Robert N. Cole, Kevin J. Yarema, Jamie B. SpanglerTBST. Next, the membrane was incubated for 1 h in streptavidin-HRP (Cell Signaling Technology, Cat 3999S) (1:150,000) and imaged. Mass spectrometry Each antibody sample was reduced and denatured and then

(19) Epidermal stratification requires retromer-mediated desmoglein-1 recyclingDevelopmental cellDecember 19, 2022Hegazy M, Koetsier JL, Huffine AL, Broussard JA, Godsel BM, Cohen-Barak E, Sprecher E, Wolfgeher DJ, Kron SJ, Godsel LM, et al.Fluor-488,Thermo Fisher Scientific,Cat# S11223 Streptavidin conjugated with HRP,Cell Signaling Technology,Cat# 3999 Pierce Streptavidin Magnetic Beads,Thermo Fisher,Cat# 88816 Dynabeads Protein G,Thermo Fisher,Cat#

(20) SH3-domain mutations selectively disrupt Csk homodimerization or PTPN22 bindingScientific ReportsDecember 1, 2022Ben F. Brian, Frances V. Sjaastad, Tanya S. Freedmanhorseradish-peroxidase-conjugated rabbit anti-goat IgG (H + L) (Southern Biotech 6164-05), Streptavidin-HRP (Cell Signaling Tech #3999), or LI-COR IRDye secondary antibodies (NC9744100, NC9523609, 926-68072) as appropriate. Blots were

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